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• Synthesis

  1. Extraction process: kelp powder → leaching → centrifugation → taking supernatant → low temperature evaporation and concentration to 1/4 of the original volume → 95% ethanol precipitation → ether degreasing → acetone decolorization → absolute ethanol washing → vacuum freeze drying → Kombu Crude Polysaccharide.
  2. Purification method: add water to dissolve kelp crude polysaccharide, and adjust the pH value to 8 with ammonia water; under the condition that the temperature is lower than 50 ℃, add 20% H2O2 dropwise until the solution is pale yellow, and then keep it for 2 hours; Distilled water was dialyzed for 3 d. After 3 d, the non-permeable part was removed by Sevag method; then dialyzed with distilled water for 3 d, added 3 times the volume of 95% ethanol for precipitation, and left standing at 4 °C overnight; centrifuged the next day, and collected the precipitate. Preliminary purified white fibrous kelp polysaccharide can be obtained by vacuum freeze-drying.
  3. Extraction and purification: Weigh kelp coarse powder and place it in a round-bottomed flask, add petroleum ether (60-90°C) to soak for 2 hours; reflux extraction for 1 hour, filter; evaporate the filter residue to dry the solvent, and extract twice with 95% ethanol under reflux , filtered for 1 h each time; the filter residue was placed in a round-bottomed flask, soaked in distilled water for 1 h, refluxed for 2 h, and filtered; then distilled water was added for reflux extraction for 2 h, filtered; the filtrates were combined, concentrated to an appropriate amount, and the protein was removed by the Sevage method. 95% ethanol was added to make the alcohol content 80%, left standing overnight, and suction filtered; the filter residue was washed several times with absolute ethanol and acetone in turn to obtain the refined polysaccharide.;

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