72-57-1

Bluish-gray to dark blue powder.

TRYPAN BLUE(72-57-1) is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. This chemical is incompatible with strong oxidizing agents and strong acids.

Azo dyes can be explosive when suspended in air at specific concentrations. Slightly soluble in water.

ACUTE/CHRONIC HAZARDS: This chemical may cause irritation and may be absorbed through the skin. It is a positive animal carcinogen. When heated to decomposition this compound emits very toxic fumes of nitrogen oxides, sulfur oxides, carbon monoxide and carbon dioxide.

Used in dyeing textiles; leather and paper; as a biological stain.

Flash point data for this chemical are not available; however, TRYPAN BLUE is probably combustible.

If this chemical gets into the eyes, remove any contact lenses at once and irrigate immediately for at least 15 minutes, occasionally lifting upper and lower lids. Seek medical attention immediately. If this chemical contacts the skin, remove contaminated clothing and wash immediately with soap and water. Seek medical attention immediately. If this chemical has been inhaled, remove from exposure, begin rescue breathing (using universal precautions, including resuscitation mask) if breathing has stopped and CPR if heart action has stopped. Transfer promptly to a medical facility. When this chemical has been swallowed, get medical attention. Give large quantities of water and induce vomiting. Do not make an unconscious person vomit.

UN2811 Toxic solids, organic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required. UN3143 dyes, solid, toxic, n.o.s. or dye intermediates, solid, toxic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required.

Dust may form explosive mixture with air. Trypan Blue is incompatible with strong oxidizing agents. Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides. Azo compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acylhalides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combinations can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Trypan Blue is sensitive to prolonged exposure to heat.

blueish grey powder

Trypan Blue is a dark Blue crystalline solid or powder.

Consult with environmental regulatory agencies for guidance on acceptable disposal practices. Generators of waste containing this contaminant (≥100 kg/mo) must conform with EPA regulations governing storage, transportation, treatment, and waste disposal.

Biological stain.

Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites.

ChEBI: An organosulfonate salt that is the tetrasodium salt of 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonic acid).

3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid(2 Moore) coupled.

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

A 0.01-0.2% aqueous solution is used for root staining. The method is often used for investigation of mycorrhizas. Suitable for whole mounts as well as sections. Also used as a stain for vitality, as living cells generally remain unstained.

blue. Blue gray powder. Soluble in water for blue, slightly soluble in soluble fiber element, insoluble in other organic solvents. The strong sulfuric acid in dark green light for blue, diluted into a red light blue; In the light of nitric acid for palm gray solution. The dye solution to join a red sulfuric acid 10% change; Add thick sodium hydroxide solution for red light purple.

Standard	Acid Resistance	Alkali Resistance	Light Fastness	Soaping		Water
				Fading	Stain	Fading	Stain
ISO	4-5	4	1-2	2		2
AATCC	5	2	1-2	2		1