6-OHDA is a catecholaminergic neurotoxin that has been used to induce dopaminergic lesions and parkinsonian symptoms as a model of Parkinson''s disease in rodents.
1 It induces locomotor impairment in mice and loss of tyrosine hydroxylase-reactive neurons in mouse brain when administered into the substantia nigra, medial forebrain bundle (MFB), or striatum at doses of 9, 6, and 18 μg, respectively.
2 Intracisternal administration of 6-OHDA (200 μg) decreases dopamine (Item No.
21992) and norepinephrine (Item No.
16673) levels in rat brain.
3 It induces apoptosis in primary rat cortical cells and PC12 rat adrenal cells when used at a concentration of 50 μM.
4,5 6-OHDA (75 μM) activates caspase-3, -8, and -9 in a time-dependent manner and increases the level of intracellular reactive oxygen species (ROS) in PC12 cells and induces apoptosis in rat substantia nigra when stereotaxically injected into the right MFB.
5,6