41994-02-9

Biotinyl tyramide (41994-02-9) is a reagent that has been used for tyramide signal amplification (TSA) via catalyzed reporter deposition (CARD). In CARD, a reporter enzyme, such as horseradish peroxidase (HRP) conjugated to a secondary antibody, is bound to the target of interest and catalyzes the covalent deposition of biotinyl tyramide to the sample. The sample is then probed by a detector, such as streptavidin-HRP, allowing detection via chromogenic or fluorescent methods. Biotinyl tyramide has been used in immunohistochemistry, ELISA, Western blot, and in situ hybridization applications.

Biotinyl Tyramide is a Biotin (B389040) derivative, a growth factor present in minute amounts in every living cell. Plays an indispensable role in numerous naturally occurring carboxylation reactions. Occurs mainly bound to proteins or polypeptides. The richest sources are liver, kidney, pancreas, yeast, and milk. The biotin content of cancerous tumors is higher than that of normal tissue.

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1) Bobrow?et al.?(1989),?Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays; J. Immunol. Methods,?125?279 2) Evans?et al.?(2003),?Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications in formalin-fixed, paraffin-embedded tissue specimens; BMC. Clin. Pathol.,?3?2 3) Draerova?et al.?(2013),?Quantification of a-tubulin by sandwich ELISA with signal amplification through niotinyl-tyramide or immune-PCR; J. Immunol. Methods,?395?63 4) Einarson and Sen (2017),?Self-biotinylation of DNA G-quadruplexes via intrinsic peroxidase activity; Nucleic Acids Res.,?45?9813 5) Hwang and Espenshade (2016),?Proximity-dependent biotin labelling in yeast using the engineered ascorbate peroxidase APEX2; Biochem. J.,?473?2463