ChemicalBook CAS DataBase List 4197-25-5

4197-25-5

Name	Solvent Black 3
CAS	4197-25-5
EINECS(EC#)	224-087-1
Molecular Formula	C29H24N6
MDL Number	MFCD00006919
Molecular Weight	456.54
MOL File	4197-25-5.mol

Synonyms

2,3-dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthyl]azo]perimidine CERES BLACK BN CI 26105 CI 26150 CI NO 26150 FAT BLACK HB LABOTEST-BB LT00451850 SOLVENT BLACK 3 SOLVENT BLACK B SUDAN BLACK B 2,3-dihydro-2,2-dimethyl-6-((4-(phenylazo)-1-naphthyl)azo)-1h-perimidin 2,3-dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthalenyl]azo]-1h-perimidin 2,3-dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthalenyl]azo]-1H-Perimidine abcolblackx60 acetylatedsudanblackb aizensotblack6 c.i.solventblack3 fastblackhbn fatblackhb01 hexatypeblackb

Chemical Properties

Appearance	VERY DARK BROWN TO BLACK FINE CRYSTALLINE POWDER
Melting point	120-124 °C(lit.)
Boiling point	552.68°C (rough estimate)
density	1.4899 (rough estimate)
refractive index	1.4570 (estimate)
storage temp.	Store at RT.
solubility	Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol
Colour Index	26150
form	Powder/Solid
pka	2.94±0.40(Predicted)
color	Very dark brown to black
Water Solubility	Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water.
ε(extinction coefficient)	≥20000 at 596-603nm in ethanol
λmax	598 nm, 415 nm
Merck	13,8970
BRN	723248
Stability:	Light Sensitive
Biological Applications	Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening
Major Application	diagnostic assay manufacturing hematology histology
Cosmetics Ingredients Functions	HAIR DYEING
InChI	1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
InChIKey	YCUVUDODLRLVIC-VPHDGDOJSA-N
SMILES	CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
CAS DataBase Reference	4197-25-5(CAS DataBase Reference)
EPA Substance Registry System	4197-25-5(EPA Substance)

Safety Data

Symbol(GHS)	GHS08
Signal word	Warning
Hazard statements	H341
Precautionary statements	P201-P202-P280-P308+P313-P405-P501
Hazard Codes	F,Xi
Risk Statements	R11:Highly Flammable. R36/37/38:Irritating to eyes, respiratory system and skin . less more
Safety Statements	S24/25:Avoid contact with skin and eyes . S16:Keep away from sources of ignition-No smoking . S37/39:Wear suitable gloves and eye/face protection . S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . less more
WGK Germany	3
RTECS	SD4431500
TSCA	Yes
HazardClass	IRRITANT
HS Code	32041900
Storage Class	11 - Combustible Solids
Hazard Classifications	Muta. 2
Toxicity	LD50 ivn-mus: 63 mg/kg CSLNX* NX#04918 less more

Raw materials And Preparation Products

Hazard Information

Chemical Properties

VERY DARK BROWN TO BLACK FINE CRYSTALLINE POWDER

Uses

Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing.

Definition

ChEBI: A member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carring a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in rozen sections and for staining of protein bound lipids in paraffin sections.

Preparation

aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidine?coupling.

Biological Activity

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reactionwith positive staining of granulocytic cellseosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smearsbut the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Safety Profile

A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx.

Staining Procedures

1 % Sudan black B is dissolved in 70% ethanol, the tissue is stained 1-1.5 h, subsequently washed in 70% ethanol (3 x 15 s), and mounted in 75% glycerol before microscopy. This staining method reveals positive results for lipids.

Properties and Applications

Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper.

Standard	Light Fastness	Heat-resistant(℃)		water	Sodium Carbonate(5%)	Hydrochloric acid(5%)
		Melting point	Stable
ISO	General	126	100	Insoluble	No change	No change

Material Safety Data Sheet(MSDS)

Spectrum Detail

Well-known Reagent Company Product Information

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