Manufacturing Process
S-Adenosyl methionine (SAM) is produced is prepared by cultivating of
Saccharomyces cerevisiae.
One loopful of each of the microorganism strains (IFO 2342, IFO 2343, IFO
2345, IFO 2346, IFO 2347) was inoculated in 10 ml of a heat-sterilized culture
medium adjusted to pH 6.0 and composed of 5.0 g/dl of glucose, 0.5 g/dl of
polypeptone, 0.4 g/dl of KH2PO4, 0.4 g/dl of K2HPO4, 0.02 g/dl of
MgSO4·7H2O and 0.2 g/dl of yeast extract, and cultivated with shaking at
28°C for 24 h.
1 L of a culture medium adjusted to pH 6.0 and composed of 10.0 g/dl of
sucrose, 1.0 g/dl of yeast extract, 0.4 g/dl of K2HPO4, 0.01 g/dl of
MgSO4·7H2O, 1.5 g/dl of urea (separately sterilized), 0.75 g/dl of Lmethionine,
0.02 g/dl of CaCl2·2H2O, 0.25 mg/dl of ZnSO4·7H2O, 0.25 mg/dl
of FeSO4·7H2O, 125.0 mg/dl of MnSO4·6H2O, 2.0 μg/dl of CuSO4·5H2O, 2.0
μg/dl of H3BO3, 0.2 μg/dl of CoCl2·6H2O and 1.0 μg/dl of KI was put in a 2-
liter fermentor and sterilized. Then, 5 ml of the seed culture broth prepared
as above was inoculated in the culture medium and cultivated at 28°C for 72
h with aeration and agitation.
After the cultivation, the microbial cells were collected by centrifugal
separation, washed once with physiological saline, suspended in 100 ml of 1.5
N perchloric acid, and shaken at room temperature for 1 h. The suspension
was then centrifuged to remove the microbial cells, and the resulting liquid
was adjusted to pH 4.5 by adding potassium hydrogen carbonate. The
resulting precipitate of potassium perchlorate was removed by centrifugal
separation to give an extract containing SAM. The amount of SAM in the
extract was determined, and the amount of SAM based on the dry cells.
The extract in an amount of 0.2 g as SAM was passed through a column filled
with 50 ml of Amberlite IRC-50 (H+ form), a weakly acidic cation exchange
resin, to cause adsorption of SAM. 0.005 N acetic acid was passed through the
column to wash it until the absorbance at 260 nm of the eluate becames less
than 0.1. Thus, impurities were removed. Then, 0.1 N sulfuric acid was
passed through the column, and SAM was eluted until the absorbance at 260
nm of the eluate becames less than 0.05. The eluate was treated with
Amberlite IRA 900 resin (OH- form) to adjust its pH to 3.0, and then
lyophilized to obtain SAM sulfate. The SAM based may be produced from SAM
sulfate by treatment with potassium hydrogen carbonate. The purity of SAM
was measured by cellulose thin-layer chromatography, paper chromatography
and high-performance liquid chromatography. The yield of SAM based on the
dry cells: IFO 2343-12.1%; IFO 2346-18.8%; IFO 2347-16.7%.
benefits
S-Adenosyl-L-methionine (SAMe) plays a role in the immune system, maintains cell membranes, and helps produce and break down brain chemicals like serotonin, melatonin, and dopamine. It works with vitamin B12 and folate (vitamin B9). Being deficient in either vitamin B12 or folate may reduce SAMe levels in your body. Several studies show that SAMe helps relieve the pain of osteoarthritis. Taking SAMe by mouth works, as well as ibuprofen and other similar drugs for reducing symptoms of osteoarthritis. Other studies suggest that SAMe may help treat depression.
