186501-28-0

Cal Green? 1 AM is a cell-permeant acetyloxy-methyl ester compound that is converted to the fluorescent calcium indicator Cal Green? 1 by intracellular esterases. This derivative is characterized by high quantum yield and low phototoxicity.¹ Its peak excitation and emission wavelengths (506 and 531 nm, respectively) are comparable to standard fluorescein dyes, making Cal Green? 1 AM appropriate for fluorescent microscopy and flow cytometry. Cal Green? 1 is ~5-fold brighter than fluo-3 at saturating calcium levels.¹

Calcium Green 1AM is a cell-permeant fluorescent calcium indicator (Excitation 506 nm; Emission 531 nm). Calcium Green 1AM is converted to the fluorescent calcium indicator by intracellular esterases[1].

1. Paredes, R.M., Etzler, J.C., Watts, L.T., et al. Chemical calcium indicators Methods 46(3),143-151(2008).