Description
Erastin2 is a ferroptosis inducer and an inhibitor of the system x
c- cystine/glutamate transporter.
1,2 It inhibits glutamate release in CCF-STTG1 cells (IC
50 = 0.0035 μM).
2 It induces cell death in HAP1 cells when used at a concentration of 5 μM, an effect that can be blocked by the ferroptosis inhibitor ferrostatin-1 or deferoxamine .
1 Erastin2 also induces ferroptotic cell death in HT-1080 cells (EC
50 = 0.15 μM), an effect that can be blocked by the reducing agent β-mercaptoethanol (EC
50 = >20 μM).
3 It increases lipid peroxidation in HT-1080 cells when used at a concentration of 1 μM.
References
1. Cao, J.Y., Poddar, A., Magtanong, L., et al. A genome-wide haploid genetic screen identifies regulators of glutathione abundance and ferroptosis sensitivity Cell Rep. 26(6),1544-1556(2019).
2. Dixon, S.J., Patel, D.N., Welsch, M., et al. Pharmacological inhibition of cystine-glutamate exchange induces endoplasmic reticulum stress and ferroptosis Elife 3,e02523(2014).
3. Magtanong, L., Ko, P.-J., To, M., et al. Exogenous monounsaturated fatty acids promote a ferroptosis-resistant cell state Cell Chem. Biol. 26(3),420-432(2019).
