150810-68-7

5-Carboxytetramethylrhodamine succinimidyl ester (5-TAMRA-SE) is an amine-reactive fluorescent probe. It has been used in double site-directed peptide modification to label proteinase substrates for use in FRET assays. It quenches lucifer yellow CH (Item No. 25573) fluorescence by greater than 90% in uncleaved proteinase substrates, which allows for the detection of enzyme-cleaved substrates by an increase in fluorescence intensity. It has also been used in the synthesis of fluorescent derivatives of a variety of compounds, including the antibiotic ampicillin (Item No. 14417), nucleotide diphosphate uridine-5’-diphosphate (UDP; Item No. 18137), and the progesterone receptor antagonist RU486 (mifepristone; Item No. 10006317). 5-TAMRA-SE displays excitation maxima ranging from 540 to 560 nm and an emission maximum of 580 nm.

Amine-Reactive Fluorescent Probe for labeling of DNA and RNA

Amine-reactive form of 5-carboxytetramethylrhodamine single isomer.

Store at -20°C

[1] KIERAN F. GEOGHEGAN. Site-directed double fluorescent tagging of human renin and collagenase (MMP-1) substrate peptides using the periodate oxidation of N-terminal serine. An apparently general strategy for provision of energy-transfer substrates for proteases[J]. Bioconjugate Chemistry Bioconjugate, 1993, 4 6: 537-544. DOI: 10.1021/bc00024a017
[2] ADAM B. SHAPIRO*  Mark S  Janelle Comita Prevoir. 5-Carboxytetramethylrhodamine-Ampicillin Fluorescence Anisotropy-Based Assay of Escherichia coli Penicillin-Binding Protein 2 Transpeptidase Inhibition[J]. ACS Infectious Diseases, 2019, 5 6: 863-872. DOI: 10.1021/acsinfecdis.8b00351
[3] JUN QI  Pablo S  Michelle Oppenheimer. Fluorescence Polarization Binding Assay for Aspergillus fumigatus Virulence Factor UDP-Galactopyranose Mutase.[J]. Enzyme Research, 2011, 2011: 513905. DOI: 10.4061/2011/513905
[4] ROY WEINSTAIN. Fluorescent Ligand for Human Progesterone Receptor Imaging in Live Cells[J]. Bioconjugate Chemistry Bioconjugate, 2013, 24 5: 766-771. DOI: 10.1021/bc3006418

553/575 nm

546 nm/580 nm

92,000

532 nm or 555 nm

532 nm or 555 nm

Alexa Fluor® 555, CF® 555, DyLight® 549