150810-68-7

5-Carboxytetramethylrhodamine succinimidyl ester (5-TAMRA-SE) is an amine-reactive fluorescent probe. It has been used in double site-directed peptide modification to label proteinase substrates for use in FRET assays. It quenches lucifer yellow CH (Item No. 25573) fluorescence by greater than 90% in uncleaved proteinase substrates, which allows for the detection of enzyme-cleaved substrates by an increase in fluorescence intensity. It has also been used in the synthesis of fluorescent derivatives of a variety of compounds, including the antibiotic ampicillin (Item No. 14417), nucleotide diphosphate uridine-5’-diphosphate (UDP; Item No. 18137), and the progesterone receptor antagonist RU486 (mifepristone; Item No. 10006317). 5-TAMRA-SE displays excitation maxima ranging from 540 to 560 nm and an emission maximum of 580 nm.

Amine-Reactive Fluorescent Probe for labeling of DNA and RNA

Amine-reactive form of 5-carboxytetramethylrhodamine single isomer.

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