Chemical Properties
White to slightly yellowish powder
Uses
β-Nicotinamide adenine dinucleotide 2′-phosphate (NADP+) and β-Nicotinamide adenine dinucleotide 2′-phosphate, reduced (NADPH) comprise a coenzyme redox pair (NADP+:NADPH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. The NAD
Description
Nicotinamide adenine dinucleotide phosphate, abbreviated NADP+ or, in older notation, TPN (triphosphopyridine nucleotide), is a cofactor used in anabolic reactions, such as the Calvin cycle and lipid and nucleic acid syntheses, which require NADPH as a reducing agent ('hydrogen source').It is used by all forms of cellular life.
Description
The nicotinamide adenine dinucleotide phosphate oxidase（NADP）is a multicomponent system that transfers an electron to molecular oxygen by way of FAD and heme to form superoxide (O2?) .Cytochrome b558 is a membrane-bound heterodimer lodged in the wall of the secondary granules; the large glycosylated β subunit is gp91phox and the small nonglycosylated α subunit is p22phox.The cytoplasmic tail of gp91phox binds FAD, heme, and NADPH, which are required for electron transfer to oxygen (O2).Neutrophil stimulation leads to aggregation and phosphorylation of p47phox, p67phox, p40phox, and the small guanosine triphosphate (GTP)–binding proteins RAC1/RAC2, which dock with the cytochrome at the membrane through binding of p47phox and p22phox.
Biosynthesis
In general, NADP+ is synthesized before NADPH is. Such a reaction usually starts with NAD+ from either the de-novo or the salvage pathway, with NAD+ kinase adding the extra phosphate group. ADP-ribosyl cyclase allows for synthesis from nicotinamide in the salvage pathway, and NADP+ phosphatase can convert NADPH back to NADH to maintain a balance.Some forms of the NAD+ kinase, notably the one in mitochondria, can also accept NADH to turn it directly into NADPH.[3][4] The prokaryotic pathway is less well understood, but with all the similar proteins the process should work in a similar way.
Biological Functions
NADP sodium salt is a coenzyme involved in cellular electron transfer reactions in biological metabolism, which is alternately oxidized (NADP+) and reduced (NADPH), and can maintain cellular redox homeostasis and regulate many biological events, including cellular metabolism. It plays a multifunctional role in regulating inflammation, redox homeostasis, and synthetic metabolism processes.
Biochem/physiol Actions
Electron acceptor
Mechanism of action
NADP sodium salt exerts its effect by competitively inhibiting nucleotide metabolism through the suppression of enzymes like nucleoside diphosphate kinase (NDPK) and adenosine kinase (ADK). Additionally, it serves as a substrate for enzymes like DNA polymerases and RNA polymerases, enabling the integration of NADP monosodium salt into nucleic acids during replication and transcription.
Synthesis
Pyridine nucleotide triphosphate, coenzyme II (NADP sodium salt) can be obtained from NADP+ by accepting the gamma-phosphate group of ATP catalyzed by a kinase.
