Cholic acid-d
4 is intended for use as an internal standard for the quantification of cholic acid (Item No.
20250) by GC- or LC-MS. Cholic acid is a primary bile acid.
1 It is formed from cholesterol
via a multistep process catalyzed by the cytochrome P450 (CYP) isoforms CYP7A1, CYP8B1, and CYP27A1. Cholic acid is conjugated to glycine or taurine by bile acid-CoA:amino acid N-acyltransferase (BAAT) to produce glycocholic acid (GCA; Item No.
20276) and taurocholic acid (TCA; Item No.
16215), respectively, in the liver, and is transformed into the secondary bile acid deoxycholic acid (DCA; Item No.
20756) by intestinal microbiota.
1,2,3 It induces
C. difficile colony formation in an agar dilution assay when used at a concentration of 0.1% w/v.
4 Dietary administration of cholic acid (0.4% w/w) increases serum cholesterol levels, biliary phospholipid secretion, and fecal DCA levels in rats.
5 
