recombinant streptavidin

Catalog Number: NRPC02A
Packing Details: 1 mg, 5 mg, 10 mg, 100 mg
Physical Appearance: Freeze-dried powder
Mol. Wt.: 16.5 kDa
Resources: Escherichia coli (E. coli)
Purity: ≥95%
Activity: ≥12 U/mg protein (Determined by NUPTEC according to modified Green Method)
Storage Condition: -20 oC
Storage Duration: 3 years
Description:
    Streptavidin is a homotetrameric protein with a molecular weight of 55 kDa found in the culture broth of Streptomyces avidinii. Similar to avidin, one mole of streptavidin can bind 4 moles of biotin with a high affinity virtually unmatched in nature. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point close to neutrality. Therefore it has a reducing nonspecific binding level as compared to avidin. Streptavidin has been extensively applied in various biological fields, such as ELISA, IHC, TRFIA, quantifying PCR, isolation of single-stranded nucleotide, purification of biomolecule, and monoclonal antibody production as well.

M：Protein Molecular Weight Marker
Lane 1：Recombinant Streptavidin subunit
Lane 2：Recombinant Streptavidin homotetramer

Notes:
    It is recommended that the product is reconstituted with steriled water into a final concentration of 1 mg/ml. Store the reconstituted product in aliquots at -20°C. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes.
    The use of strong acids and alkalis, strong oxidants, and high concentrations of organic solvents should be avoided to protect the product from denaturation.