汇智和源生物技术(苏州)有限公司

近日，司法鉴定科学研究院严慧课题组汤以绫老师，使用IPHASE品牌产品：II相代谢稳定性试剂盒（UGTs）在《Journal of Pharmaceutical and Biomedical Analysis》权威期刊上发表文章《Metabolism of dipentylone in zebrafish and human liver microsomes determined by liquid chromatography–high resolution mass spectrometry 》，影响因子3.4！

本论文中，利用LC-HRMS分析dipentylone在斑马鱼和HLM体内和体外的代谢，并成功鉴定了代谢产物及其主要代谢途径，选择了合适的代谢物作为生物标志物。

摘要

The consumption of novel psychoactive substances (NPS) is exceedingly prevalent in society, as these substances are sold and distributed as “legal highs.” One novel synthetic cathinone emerging in the market is 1-(1,3-ben-zodioxol-5-yl)–2-(dimethylamino) pentan-1-one (dipentylone). The goal of this work was to study the in vivo and in vitro metabolism of dipentylone in zebrafish and human liver microsomes (HLMs) by liquid chromatography-high resolution mass spectrometry (LCHRMS). The zebrafish and HLM samples contained 14 dipentylone metabolites, specifically 12 phase I metabolites and 2 phase II metabolites. The main metabolic pathways included monohydroxylation (M1 and M2), N-dealkylation (M3), hydroxylation of the aromatic ring and dealkoxylation of M3 (M4), O-dealkylation (M5), N-dealkylation of M5 (M6), reduction of carboxide (M7), monohydroxylation of M5 (M8), dehydrogenation (M9), dealkoxylation (M10), N-dealkylation of M10 (M11), dealkoxylation of M9 (M12), glucuronidation of M5 (M13), and sulfation (M14). The monohydroxylated metabolite (M2) can be recommended as metabolic markers for dipentylone. This study is the first to identify a target compound for monitoring the abuse of dipentylone and to determine the essential chemical structure of the metabolites for urther toxicological research.

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