SC-43 (0-10 μM; 24-72 hours; HuCCT-1, KKU-100, and CGCCA cells) treatment reveals the anti-proliferative effects in cholangiocarcinoma (CCA) cell lines in a dose-dependent manner after treating 24, 48 and 72 hours respectively.
Its treatment shows increased sub-G1 cells and G2-M arrest, indicating SC-43 induced differential apoptotic effects in these cell lines.
Its (0-10 μM; 24 hours; HuCCT-1, KKU-100, and CGCCA cells) treatment demonstrates significant increase in cleaved caspase-3 and PARP level.
Its activates SH2 domain-containing phosphatase 1 (SHP-1) activity, leading to p-STAT3 and downstream cyclin B1 and Cdc2 downregulation. Its augments SHP-1 activity by direct binding to N-SH2 and relief of its autoinhibition.
Cell Cycle Analysis
|
Cell Line:
|
HuCCT-1, KKU-100, and CGCCA cells
|
|
Concentration:
|
0 μM, 1 μM, 2.5 μM, 5 μM, 10 μM
|
|
Incubation Time:
|
24 hours
|
|
Result:
|
Showed increased sub-G1 cells and G2-M arrest.
|
Western Blot Analysis
|
Cell Line:
|
HuCCT-1, KKU-100, and CGCCA cells
|
|
Concentration:
|
0 μM, 1 μM, 2.5 μM, 5 μM, 10 μM
|
|
Incubation Time:
|
24 hours
|
|
Result:
|
Demonstrated significant increase in cleaved caspase-3 and PARP level.
|
