α-Zearalenol 是一种对雌激素受体 (ER) 具有高亲和力的霉菌毒素,
α-Zearalenol 是 zearalenone (ZEN) 的衍生物，由于其异种雌激素效应可引起动物的生殖障碍。
α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) shows the highest RPE (90.5%) at 9.37 µM α-ZOL exposure in MFC-7 cells with an IC
50
of 12.5 μM.
α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) induces an increase of cell viability on MCF-7 cells mainly after 72 h of treatment whereas it is slightly cytotoxic on MDA-MB231 cells.
α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) increases MCF7 cell population in the S phase and in the G2/M phase, no visible change in cell cycle phases is observed in MDA-MB231 cells.
α-Zearalenol (α-zol) (1-10 μM; 24 hours) reduces the expression of TNF-α, α-ZOL combines with β-ZOL shows an antagonistic effect on inflammation for IL-1β and act synergic for IL-8 at high toxin concentrations.
Cell Proliferation Assay
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Cell Line:
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MFC-7 cells
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Concentration:
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6.25 µM-25 µM
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Incubation Time:
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24 hours
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Result:
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Observed the highest RPE (90.5%) at 9.37 µM α-ZOL exposure in MFC-7 cells.
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Cell Viability Assay
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Cell Line:
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Breast cancer cell lines: MCF-7 and MDA-MB231
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Concentration:
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0.001 μM, 0.1 μM and 10 μM
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Incubation Time:
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24 hours, 48 hours, 72 hours
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Result:
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Modified the cell growth in two breast cancer cell lines.
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Cell Cycle Analysis
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Cell Line:
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MCF7 cells, MDA-MB231 cells
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Concentration:
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0.001 μM, 0.1 μM and 10 μM
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Incubation Time:
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72 hours
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Result:
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Observed an increase of cells in cell cycle in MCF7 cells.
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RT-PCR
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Cell Line:
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HepG2 cells
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Concentration:
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1 μM, 5 μM, 10 μM
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Incubation Time:
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72 hours
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Result:
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Induced a decrease of the expression of TNF-α, IL-1β and IL-8, that was in most cases statistically significant for the higher doses.
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