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外観
暗緑色又は黒褐色, 粉末又は塊
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溶解性
水 (4.0%), エタノール (1.8%), セロソルブ (3.75%), エチレングリコール (3.0%), クロロホルムに可溶。エチルエーテル, キシレンにほとんど溶けない。水にやや溶けやすく、エタノールにやや溶けにくい。
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解説
3-amino-7-dimethylamino-2-methylphenazine hydrochloride．C15H17ClN4(288.78)．m-トルイレンジアミンにp-ニトロソジメチルアニリンを作用させ，得られたインダミンを酸化すると得られる．暗緑色の粉末．水，エタノールに可溶．pH 6.8で赤色，pH 8.0で黄色を呈する．酸塩基指示薬．0.1％ の60％ エタノール溶液は，水，尿素，亜硝酸などのアルカリ度を測定するための滴定指示薬に用いられる．ニュートラルレッド紙，生細胞の生化学的染色に利用される．森北出版「化学辞典（第2版）
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用途
有機合成原料。
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化学的特性
dark green powder
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使用
Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites.
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主な応用
Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.
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定義
ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+).
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製造方法
(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.
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一般的な説明
Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.
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Staining Procedures
Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.
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純化方法
In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]
