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定義
本品は、枯草菌 Bacillus subtilis 又は Bacillus licheniformis から得られる酵素である。
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化粧品の成分用途
酵素剤、皮膚コンディショニング剤
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化学的特性
These are proteolytic enzymes which take the form of light-colored, free-flowing powders. A protein containing numerous amino acids
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使用
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).
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一般的な説明
Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.
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安全性プロファイル
Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits toxicfumes of NOx.
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職業ばく露
These commercial proteolytic enzymes are used in laundry detergent formulations
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純化方法
This alkaline protease is purified 211-fold by affinity chromatography using 4-(4-aminophenylazo)phenylarsonic acid complex to activated CH-Sepharose 4B. It is inhibited by 2-phenylethane boronic acid, PMSF, 3,4-dichloroisocoumarin, acetone and benzamide. [Chandraskaren & Dhar Anal Biochem 150 141 1985, Schomburg & Schomburg Springer Handbook of Enzymes 2nd Edn vol 7 p 286 2002.] Synexin (from bovine liver) M 47,000 Da. This Ca binding protein is purified by (NH4)2SO4 precipitation, then by a specific pH step elution from a chromatofocusing medium in the absence of ampholytes. The pI is 7.5. [Scott et al. Anal Biochem 149 163 1985.]
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不和合性
Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides.
