ALPHA-玉米赤霉烯醇

生物活性体外研究 ALPHA-玉米赤霉烯醇试剂级价格

ALPHA-玉米赤霉烯醇

CAS号:36455-72-8
英文名:ALPHA-ZEARALENOL
中文名:ALPHA-玉米赤霉烯醇
CBNumber:CB8491950
分子式:C18H24O5
分子量:320.38
MOL File:36455-72-8.mol

ALPHA-玉米赤霉烯醇化学性质

熔点 :158-161°C
沸点 :599.0±50.0 °C(Predicted)
密度 :1.174±0.06 g/cm3(Predicted)
闪点 :2℃
储存条件 :-20°C
溶解度 :≤20mg/ml in ethanol;30mg/ml in DMSO;30mg/ml in dimethyl formamide
形态 :crystalline solid
酸度系数(pKa) :7.61±0.60(Predicted)
稳定性 :Hygroscopic

安全信息

危险品标志 :Xn,T,F
危险类别码 :20/21/22-40-52/53-68/20/21/22-36-11-62-48/20-63-46-45
安全说明 :36/37-61-16-45-53
危险品运输编号 :UN 1648 3 / PGII
WGK Germany :3
F :8
海关编码 :29322090

ALPHA-玉米赤霉烯醇性质、用途与生产工艺

生物活性 α-Zearalenol 是一种对雌激素受体 (ER) 具有高亲和力的霉菌毒素, α-Zearalenol 是 zearalenone (ZEN) 的衍生物，由于其异种雌激素效应可引起动物的生殖障碍。

体外研究

α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) shows the highest RPE (90.5%) at 9.37 µM α-ZOL exposure in MFC-7 cells with an IC ₅₀ of 12.5 μM. α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) induces an increase of cell viability on MCF-7 cells mainly after 72 h of treatment whereas it is slightly cytotoxic on MDA-MB231 cells. α-Zearalenol (α-zol) (0.001-10 μM; 24-72 hours) increases MCF7 cell population in the S phase and in the G2/M phase, no visible change in cell cycle phases is observed in MDA-MB231 cells. α-Zearalenol (α-zol) (1-10 μM; 24 hours) reduces the expression of TNF-α, α-ZOL combines with β-ZOL shows an antagonistic effect on inflammation for IL-1β and act synergic for IL-8 at high toxin concentrations.

Cell Proliferation Assay

Cell Line:	MFC-7 cells
Concentration:	6.25 µM-25 µM
Incubation Time:	24 hours
Result:	Observed the highest RPE (90.5%) at 9.37 µM α-ZOL exposure in MFC-7 cells.

Cell Viability Assay

Cell Line:	Breast cancer cell lines: MCF-7 and MDA-MB231
Concentration:	0.001 μM, 0.1 μM and 10 μM
Incubation Time:	24 hours, 48 hours, 72 hours
Result:	Modified the cell growth in two breast cancer cell lines.

Cell Cycle Analysis

Cell Line:	MCF7 cells, MDA-MB231 cells
Concentration:	0.001 μM, 0.1 μM and 10 μM
Incubation Time:	72 hours
Result:	Observed an increase of cells in cell cycle in MCF7 cells.

RT-PCR

Cell Line:	HepG2 cells
Concentration:	1 μM, 5 μM, 10 μM
Incubation Time:	72 hours
Result:	Induced a decrease of the expression of TNF-α, IL-1β and IL-8, that was in most cases statistically significant for the higher doses.