CPTH2

生物活性靶点体外研究 CPTH2 试剂级价格

CPTH2

CAS号:357649-93-5
英文名:Histone Acetyltransferase Inhibitor IV, CPTH2
中文名:CPTH2
CBNumber:CB22602855
分子式:C14H14ClN3S
分子量:291.8
MOL File:357649-93-5.mol

CPTH2化学性质

熔点 :190-191 °C
沸点 :460.3±37.0 °C(Predicted)
密度 :1.38±0.1 g/cm3(Predicted)
储存条件 :2-8°C
溶解度 :Soluble in DMSO
形态 :powder
酸度系数(pKa) :14.57±0.20(Predicted)
颜色 :white to beige
水溶解性 :Water: < 0.1 mg/mL (insoluble)
敏感性 :Light Sensitive
稳定性 :Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 month.

安全信息

WGK Germany :nwg

CPTH2性质、用途与生产工艺

生物活性 CPTH2 是一种有效的 histone acetyltransferase (HAT) 的抑制剂，可调节Gcn5p网络。CPTH2 通过抑制 KAT3B 诱导凋亡并降低ccRCC细胞系的侵袭性。
靶点
Target Value
HAT
()
Gcn5p
()
KAT3B
()

体外研究

CPTH2 (100 μM; 12, 24, 48 hours) causes a decrease in cell proliferation after as early as 12 h with a further significant reduction after 48 h stimulation.
CPTH2 (100 μM; 12 or 48 hours) causes a comparable drop of the activity in both cell lines.
CPTH2 (100 μM; 48 hours) produces a drastic increase in apoptotic/dead cell population after 48 h.
CPTH2 (100 μM; 12, 24, 48 hours) shows a reduced acetylation of both global AcH3 histone and H3AcK18.
CPTH2 (100 μM; 24, 48 hours) is capable to counteract invasion and migration of ccRCC-786-O cells in culture.
CPTH2 (0.2, 0.5, 1 mM) inhibits the growth of a GCN5 deleted strain and a single catalytic mutant E173H.
CPTH2 (0.6, 0.8 mM; for 24 hours) inhibits histone H3 acetylation in yeast cell cultures.
CPTH2 inhibits the Gcn5p dependent functional network.

Cell Proliferation Assay

Cell Line:	Papillary thyroid (K1) and clear cell Renal Cell Carcinoma (ccRCC-786-O) cell lines
Concentration:	100 μM
Incubation Time:	12, 24, 48 hours
Result:	Caused a decrease in cell proliferation after as early as 12 h with a further significant reduction after 48 h stimulation.

Cell Viability Assay

Cell Line:	K1 and ccRCC-786-O cell lines
Concentration:	100 μM
Incubation Time:	24 hours (K1 cell) and 48 hours (ccRCC-786-O cell)
Result:	Caused a comparable drop of the activity in both cell lines.

Apoptosis Analysis

Cell Line:	ccRCC-786-O cells
Concentration:	100 μM
Incubation Time:	48 hours
Result:	Produced a drastic increase in apoptotic/dead cell population after 48 h.

Western Blot Analysis

Cell Line:	ccRCC-786-O cells
Concentration:	100 μM
Incubation Time:	12, 24, 48 hours
Result:	Showed a reduced acetylation of both global AcH3 histone and H3AcK18.