4-羟基壬烯醛

生物活性靶点体外研究体内研究 4-羟基壬烯醛试剂级价格

4-羟基壬烯醛

CAS号:75899-68-2
英文名:HNE
中文名:4-羟基壬烯醛
CBNumber:CB0281140
分子式:C9H16O2
分子量:156.22
MOL File:75899-68-2.mol

4-羟基壬烯醛化学性质

沸点 :125-127 °C(Press: 2 Torr)
密度 :0.941±0.06 g/cm3(Predicted)
储存条件 :-70°C
溶解度 :DMF: >50 mg/ml; DMSO: >50 mg/ml; Ethanol: >50 mg/ml; PBS pH 7.2: >1 mg/ml
酸度系数(pKa) :13.31±0.20(Predicted)
形态 :Colorless oil.
CAS 数据库 :75899-68-2(CAS DataBase Reference)

4-羟基壬烯醛性质、用途与生产工艺

生物活性 4-Hydroxynonenal (4-HNE) 是一种 α，β 不饱和羟基烯醛，是一种氧化/亚硝化应激生物标志物。4-Hydroxynonenalal 是 ALDH2 的底物和抑制剂。4-Hydroxynonenalal 可以调节许多信号传导过程，主要是通过与蛋白质，核酸和膜脂质中具有亲核官能团的共价加合物形成的。4-Hydroxynonenalal 通过线粒体在癌症中起重要作用。
靶点

Human Endogenous Metabolite
体外研究

4-Hydroxynonenal is both a substrate and an inhibitor of ALDH2; inhibition of ALDH2 by 4-Hydroxynonenal is reversible at low concentration and become irreversible when the concentration of 4-HNE reaches 10 µM.
4-Hydroxynonenal can induce antioxidant defense mechanisms to restrain its own production and to enhance the cellular protection against oxidative stress.
4-Hydroxynonenal, the product of lipid peroxidation, is mutagenic and genotoxic in viruses, bacteria and mammalian cells. It reacts with all four DNA bases but with different efficiency: G >C > A >T. 4-Hydroxynonenal-dG represents the best biomarker of the genotoxic effects of 4-Hydroxynonenal and these adducts are primarily found in nuclear DNA. A classic example of etiological relevance of 4-Hydroxynonenal-dG in human cancers is 4-Hydroxynonenal-dG induced p53 mutation. 4-Hydroxynonenal-dG adducts were preferentially formed at the third base of codon 249 in the p53 gene, causing gene mutation and affecting diverse biological processes including cell cycle arrest, apoptosis, DNA repair, and differentiation.
体内研究

Following 24 h after fluid percussion injury (FPI), the mouse brain tissue is analyzed for the expression level of NADPH oxidase 1 (NOX1), inducible nitric oxide synthase (iNOS), 4-Hydroxynonenal (4-HNE. Both wild-type (Nrf2 ^+/+ ) and Nrf2-deficient mice (Nrf2 ^−/− ) results in increased expression of 4-Hydroxynonenal following 15 psi injury (moderate injury) when compared to uninjured Nrf2 ^+/+ and Nrf2 ^−/− mice. Similar to iNOS result, in Nrf2 ^−/− KO mice, the expression level of 4-Hydroxynonenal is significantly high when compared to corresponding injured and uninjured Nrf2 ^+/+ WT animals.